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Multiple‐axis tomography: applications to basal bodies from Paramecium tetraurelia
Author(s) -
Messaoudi Cédric,
Loubresse Nicole Garreau,
Boudier Thomas,
DupuisWilliams Pascale,
Marco Sergio
Publication year - 2006
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1042/bc20050097
Subject(s) - tilt (camera) , tomography , electron tomography , wedge (geometry) , computer science , computer vision , imaging phantom , biology , physics , optics , artificial intelligence , transmission electron microscopy , scanning transmission electron microscopy , geometry , mathematics
Background information . Transmission electron tomography is becoming a powerful tool for studying subcellular components of cells. Classical approaches for electron tomography consist of recording images along a single‐tilt axis. This approach is being improved by dual‐axis reconstructions and/or high‐tilt devices (tilt angle>±60°) on microscopes to compensate part of the information loss due to the ‘missing wedge’ phenomena. Results . In the present work we have evaluated the extension of the dual‐axis technique to a multiple‐axis approach, and we demonstrate a freely available plug‐in for the Java‐based freeware image‐analysis software ImageJ. Our results from phantom and experimental data sets from Paramecium tetraurelia epon‐embedded sections have shown that multiple‐axis tomography achieves results equivalent to those obtained by dual‐axis approach without the requirement for high‐tilt devices. Conclusions . This new approach allows performance of high‐resolution tomography, avoiding the need for high‐tilt devices, and therefore will increase the access of electron tomography to a larger community.