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Yeast Ypt11 is targeted to recycling endosomes in mammalian cells
Author(s) -
Kail Mark,
Hollinshead Mike,
Kaufmann Miriam,
Boettcher Jens,
Vaux David,
Barnekow Angelika
Publication year - 2005
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1042/bc20040139
Subject(s) - endosome , green fluorescent protein , brefeldin a , biology , microbiology and biotechnology , golgi apparatus , fusion protein , compartment (ship) , transport protein , mcherry , vesicle , heterologous , fluorescence microscope , protein targeting , budding , fluorescence , endoplasmic reticulum , biochemistry , membrane protein , gene , membrane , intracellular , recombinant dna , oceanography , physics , quantum mechanics , geology
Background information . In yeast, Ypt11 or Ypt32 along with the highly homologous Ypt8 or Ypt31 has been reported to be an essential component of intra‐Golgi trafficking and has been implicated in the budding of vesicles from the most distal Golgi compartment. Results and conclusions . In the present study, we show that, in human cells, after heterologous expression of GFP—Ypt11 (where GFP stands for green fluorescent protein), the protein is targeted to transferrin‐positive recycling endosomes. This compartment has been shown to form extensive tubular networks on applying the drug Brefeldin A. We also show, by confocal fluorescent microscopy, that these networks also contain Rab11 in cells expressing CFP—Rab11a (where CFP stands for cyan fluorescent protein) fusion protein and that these structures are identical with those targeted by GFP—Ypt11.