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Heat‐shock response down‐regulates interleukin‐18 expression in murine peritoneal macrophages
Author(s) -
Wang Yun,
Chang Zongliang
Publication year - 2005
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1042/bc20040108
Subject(s) - biology , microbiology and biotechnology , heat shock , interleukin 1β , shock (circulatory) , immunology , interleukin , heat shock protein , cytokine , medicine , biochemistry , gene
Background information . The heat‐shock response is a self‐defence mechanism that protects cells and organisms from a wide range of harmful stresses. Recent studies revealed that it involved the regulation of cytokine expression. Interleukin‐18 (IL‐18) is an important cytokine in mediating immune response. Results . We studied interferon‐γ (IFN‐γ)‐induced IL‐18 expression in heat‐shock‐treated murine peritoneal macrophages. Our results showed that the heat‐shock response significantly inhibited the expression of IFN‐γ‐induced pro‐inflammatory cytokine IL‐18. Interferon consensus sequence binding protein (ICSBP) is a transcription factor that binds to the promoter of IL‐18 and regulates the transcription of IL‐18. Further research on the down‐regulation mechanism showed that the DNA‐binding activity of ICSBP was greatly reduced by the heat shock response. Conclusions . These results suggest that the inhibitory effect of heat‐shock response on IL‐18 production in IFN‐γ‐stimulated macrophages is related to the suppression of the binding activity of ICSBP.