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Cyclic mechanical strain promotes transforming‐growth‐factor‐β1‐mediated cardiomyogenic marker expression in bone‐marrow‐derived mesenchymal stem cells in vitro
Author(s) -
Bhang Suk Ho,
Gwak SoJung,
Lee TaeJin,
Kim SangSoo,
Park Ho Hyun,
Park Moon Hyang,
Lee DaeHee,
Lee SooHong,
Kim ByungSoo
Publication year - 2010
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20090307
Subject(s) - mesenchymal stem cell , transforming growth factor , in vitro , microbiology and biotechnology , chemistry , stem cell , bone marrow , biology , immunology , biochemistry
Cardiomyocytes in the heart reside in mechanically dynamic environments, such as those subject to cyclic mechanical strain. TGF‐β1 (transforming growth factor‐β1) stimulates cardiomyogenic marker expression of BMMSCs (bone‐marrow‐derived mesenchymal stem cells). In the present study, we tested the hypothesis that cyclic mechanical strain promotes TGF‐β1‐mediated cardiomyogenic marker expression in BMMSCs in vitro . The mRNA expression of cardiac‐specific genes was more up‐regulated in BMMSCs cultured with a TGF‐β1 supplement and subjected to cyclic strain for 1 week than in BMMSCs cultured statically with a TGF‐β1 supplement. Immunocytochemical analyses and flow cytometric analysis showed that the proportions of cardiac troponin‐I‐positive cells and cardiac MHC (myosin heavy chain)‐positive cells and the proportions of cells expressing tropomyosin respectively were increased to a greater extent by TGF‐β1with cyclic strain than by TGF‐β1 alone. These results showed that cyclic strain promotes TGF‐β1mediated cardiomyogenic marker expression in BMMSCs in vitro .