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Cell‐surface display of the active mannanase in Yarrowia lipolytica with a novel surface‐display system
Author(s) -
Yang XiaoSong,
Jiang ZhengBing,
Song HuiTing,
Jiang SiJing,
Madzak Catherine,
Ma LiXin
Publication year - 2009
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20090222
Subject(s) - yarrowia , recombinant dna , yeast , bacillus subtilis , saccharomyces cerevisiae , biochemistry , chemistry , fermentation , cell wall , biology , bacteria , genetics , gene
A novel surface‐display system was constructed using the cell‐wall anchor protein Flo1p from Saccharomyces cerevisiae , the mannanase (man1) from Bacillus subtilis fused with the C‐terminus of Flo1p and the 6×His tag was inserted between Flo1p and man1. The fusion protein was displayed on the cell surface of Yarrowia lipolytica successfully, and it was confirmed by immunofluorescence. In succession, the surface‐displayed mannanase was characterized. The optimum catalytic conditions for the recombinant mannanase were 55 °C at pH 6.0, and it exhibited high stability against pH variation. The highest activity of the recombinant mannanase reached 62.3 IU/g (dry cell weight) after the recombinant was cultivated for 96 h in YPD medium [1% (w/v) yeast extract/2% (w/v) peptone/2% (w/v) glucose]. To our knowledge, the present paper is the first to report that high‐activity mannanase is displayed on the cell surface of Y. lipolytica with Flo1p.