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Efficient regioselective synthesis of 3′‐ O ‐crotonylfloxuridine catalysed by Pseudomonas cepacia lipase
Author(s) -
Zhao Zhilei,
Zong Minhua,
Li Ning
Publication year - 2009
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20080018
Subject(s) - acylation , regioselectivity , lipase , chemistry , pseudomonas , isomerization , catalysis , organic chemistry , substrate (aquarium) , triacylglycerol lipase , enzyme , bacteria , biology , ecology , genetics
Pseudomonas cepacia lipase‐catalysed preferential acylation of the secondary hydroxy group of FUdR (floxuridine) with vinyl crotonate was carried out in spite of the presence of the primary hydroxy group, and 3′‐ O ‐crotonylfloxuridine was prepared successfully for the first time. The isomerization of the double bond of crotonate, which occurs in conventional organic synthesis, could be effectively avoided during the enzymatic acylation. The effects of some key factors such as reaction medium, initial a w (water activity), molar ratio of vinyl crotonate to FUdR, FUdR concentration and reaction temperature on the reaction were examined. Under the optimized reaction conditions, the initial reaction rate, substrate conversion and 3′‐regioselectivity of the reaction were as high as 24 mM/h, 98% and 85% respectively.