Premium
The antibacterial efficacy of an aceraceous plant [Shantung maple ( Acer truncatum Bunge)] may be related to inhibition of bacterial β‐oxoacyl‐acyl carrier protein reductase (FabG)
Author(s) -
Zhang Feng,
Luo ShiYun,
Ye YanBin,
Zhao WenHua,
Sun XuGuang,
Wang ZhiQun,
Li Ran,
Sun YingHui,
Tian WeiXi,
Zhang YingXia
Publication year - 2008
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20070255
Subject(s) - polyphenol , antibacterial activity , chemistry , biochemistry , reductase , enzyme , bacteria , antimicrobial , biology , botany , traditional medicine , antioxidant , organic chemistry , medicine , genetics
Polyphenols, including flavonoids, are the major components of the extracts from aceraceous plants. They possess remarkable antibacterial and antitumour activity. Our study focused on whether the inhibition of the bacterial type II fatty acid synthesis system is the mechanism for the antibacterial effect of the related plant polyphenols. Extracts obtained from the fallen leaves of the Shantung maple ( Acer truncatum Bunge) using different solvents, and the related pure compound PGG (1,2,3,4,6‐penta‐ O ‐galloyl‐β‐ d ‐glucose), potently inhibited the FabG (β‐oxoacyl‐ACP reductase) steps in the fatty‐acid‐elongation cycle with the IC 50 values between 0.9 and 7.2 μg/ml. An ethyl acetate extract appeared to inhibit FabG reductase in a mixed manner with NADPH, as did PGG with NADPH, demonstrating that they interfered with the binding of the cofactor to the enzyme. Gram‐positive and Gram‐negative bacteria and some fungi were used to evaluate the antibacterial abilities of different extract samples. The experiments showed that a higher polyphenol content of the extracts led to a more potent inhibitory capacity against FabG, thus enhancing the antibacterial efficacy.