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Chitosan as a carrier for non‐viral gene transfer in a cystic‐fibrosis cell line
Author(s) -
Nydert Per,
Dragomir Anca,
Hjelte Lena
Publication year - 2008
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20070197
Subject(s) - transfection , hek 293 cells , cell culture , chitosan , green fluorescent protein , cell , cystic fibrosis , microbiology and biotechnology , gene delivery , reporter gene , chemistry , biophysics , biology , gene , gene expression , biochemistry , genetics
The gene transfer mediated by chitosan in CFBE41o − (a cystic‐fibrosis bronchial epithelial cell line) and HEK (a human embryonic kidney cell line) has been evaluated. Polyplexes based on chitosan and PEI (polyethyleneimine) using a luciferase and enhanced green fluorescent protein reporter plasmid showed that the transfection efficacy of polyplexes in the CFBE41o − cell line was poor compared with that in HEK cells. In the highly differentiated cystic‐fibrosis bronchial epithelial cell line the narrow‐size‐distributed chitosan shows enhanced transfection at a low pH compared with PEI. The enhanced transfection at lower pH could be a result of damage to the cell surface or changes in the cell‐surface charge, leading to better penetration of the cell membrane.