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Three‐dimensional organization of dermal fibroblasts by macromass culture
Author(s) -
Deshpande Manisha
Publication year - 2008
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20070120
Subject(s) - scaffold , in vitro , cell culture , microbiology and biotechnology , tissue culture , matrix (chemical analysis) , seeding , tissue engineering , centrifugation , biophysics , chemistry , biology , biomedical engineering , biochemistry , genetics , engineering , chromatography , agronomy
The three‐dimensional organization of cells by high‐cell‐seeding‐density culture, termed ‘macromass culture’, is described. By macromass culture, dermal fibroblasts can be made to organize themselves into a unified three‐dimensional form without the aid of a scaffold, and macroscopic constructs, named macromasses, can be made wholly from cells. The sole factor causing three‐dimensional organization is culture of cells at high cell seeding density per unit area. No scaffold or extraneous matrix is used for the generation of macromasses; they are of completely cellular origin. No other agents or external influences such as tissue‐inducing chemicals, tissue‐inducing growth factors, substratum with special properties, rotational culture, centrifugation etc. are employed for macromass formation, and all seeded cells become part of the cohesive construct. These three‐dimensional constructs have the potential for use as in vitro tissue analogues, and a possible application for in vitro cytotoxicity testing is demonstrated.