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RNA‐interference‐mediated Cdc42 silencing down‐regulates phosphorylation of STAT3 and suppresses growth in human bladder‐cancer cells
Author(s) -
Wu Fuguo,
Chen Yirong,
Li Yuan,
Ju Jun,
Wang Zhiping,
Yan Dongwen
Publication year - 2008
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20070107
Subject(s) - rna interference , gene silencing , cdc42 , cancer research , small hairpin rna , biology , small interfering rna , cell cycle , cancer cell , cell growth , microbiology and biotechnology , transfection , cell culture , cancer , kinase , gene knockdown , rna , gene , genetics
Cdc42 (cell division cycle 42), a member of Rho GTPases, is involved in cell transformation, proliferation, survival, invasion and metastasis of human cancer cells. Here, RNAi (RNA interference)‐mediated gene silencing was used to investigate the roles of Cdc42 and to assess its therapeutic potential in human bladder cancer. The results showed that Cdc42 silencing resulted in a marked reduction of Cdc42 mRNA and protein expression and a significant inhibition of cell proliferation from G 0 /G 1 ‐ to S‐phase in two (EJ and T24) human bladder‐cancer cell lines. Moreover, RNAi‐mediated inhibition of Cdc42 induced apoptosis of EJ cells 96 h after transfection. In addition, we found that silencing of Cdc42 could down‐regulate the level of phosphorylated STAT3 (signal transducer and activator of transcription 3), but did not influence the level of total STAT3 in the two bladder‐cancer cell lines. These results suggest that RNAi‐mediated Cdc42 silencing may be a novel approach for gene therapy of bladder cancer.