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Functional expression of human‐epidermal‐growth‐factor receptor in a melanoma cell line
Author(s) -
Diaz Arlhee,
Suarez Eduardo,
Blanco Rances,
Badia Teresita,
Rivero Dianelys,
LopezRequena Alejandro,
Lopez Armando,
Montero Enrique
Publication year - 2007
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20070009
Subject(s) - epidermal growth factor receptor , transfection , cell culture , monoclonal antibody , biology , melanoma , cancer research , epidermal growth factor , receptor , immunohistochemistry , cell growth , antibody , microbiology and biotechnology , immunology , genetics
EGFR [EGF (epidermal growth factor) receptor] overexpression correlates with poor prognosis and bad outcomes in different tumours. However, evidence for EGFR contribution in melanoma immunobiology is limited. We have expressed the full‐length human EGFR gene in a murine melanoma cell line. EGFR protein expression in stably trnasfected B16 cells in culture was defined by immunoblotting, immunohistochemistry and FACS. Additionally, transfected cells became sensitive to the lysis induced with an anti‐EGFR monoclonal antibody in the presence of complement. Exogenous human EGF addition induced cell proliferation, validating the transfected receptor functionality. Thus we have developed a system to express a functional EGFR in order to evaluate the potential contribution of EGFR expression in melanoma biology and its resulting relevance as a target for immunointerventions in nonepithelial tumours.