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Expression of human acidic fibroblast growth factor in Nicotiana benthamiana with a potato‐virus‐X‐based binary vector
Author(s) -
Liu Jingying,
Ma Pengda,
Sun Yan,
Yang Meiying,
Yang Liping,
Li Yuxi,
Wu Yanfang,
Zhu Xiaojuan,
Wang Xingzhi
Publication year - 2007
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20070004
Subject(s) - nicotiana benthamiana , potato virus x , recombinant dna , potexvirus , biology , microbiology and biotechnology , tobacco etch virus , viral vector , expression vector , virus , virology , biochemistry , plant virus , gene , potyvirus , rna , coat protein
The aFGF (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. A DNA fragment encoding haFGF (human aFGF) has been cloned into the PVX (potato virus X)‐based binary vector (pgR107) and transiently expressed in Nicotiana benthamiana (a wild Australian tobacco) by agroinfection. Approx. 1 week after agroinfection, the recombinant haFGF accumulated in the agroinfected plants reached up to 1% of the total soluble protein. haFGF was then purified on heparin–Sepharose CL‐6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically.