Premium
Sulfite reductase‐mediated synthesis of gold nanoparticles capped with phytochelatin
Author(s) -
Kumar Suresh Anil,
Abyaneh Majid Kazemian,
Gosavi Suresh W.,
Kulkarni Sulabha K.,
Ahmad Absar,
Khan M. Islam
Publication year - 2007
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20060205
Subject(s) - colloidal gold , sulfite , sulfite reductase , nanoparticle , nanomaterials , hydrosol , chemistry , x ray photoelectron spectroscopy , reductase , combinatorial chemistry , nanotechnology , nuclear chemistry , materials science , enzyme , inorganic chemistry , chemical engineering , organic chemistry , engineering
An enzymatic synthesis route to peptide‐capped gold nanoparticles has been developed. Gold nanoparticles were synthesized using α‐NADPH‐dependent sulfite reductase and phytochelatin in vitro . The gold ions were reduced in the presence of the enzyme sulfite reductase, leading to the formation of a stable gold hydrosol of dimensions 7–20 nm and were stabilized by the capping peptide. The nanoparticles were characterized by X‐ray diffraction, transmission electron microscopy, X‐ray photoelectron spectroscopy and UV–visible optical absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical compositions, shapes and sizes as well as their separation.