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Recombinant‐protein production in insect cells utilizing a hollow‐fibre bioreactor
Author(s) -
Baxter Sabine C.,
Panarello Dominick,
Ajith Sandya,
Bhattacharya Madhushree,
Chaiken Irwin M.
Publication year - 2006
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20060074
Subject(s) - recombinant dna , bioreactor , schneider 2 cells , drosophila melanogaster , microbiology and biotechnology , biology , extracellular , downstream processing , cell culture , biochemistry , gene , botany , genetics , rna , rna interference
Here, we demonstrate for the first time that the hollow‐fibre bioreactor is an excellent tool for the production of Drosophila ‐expressed recombinant proteins. Using the example of the soluble extracellular portion of the human IL‐5 (interleukin 5) receptor α expression in S2 (Schneider's Drosophila melanogaster cell line 2) cells, we found that it is possible to produce multi‐milligram amounts of functional recombinant protein continuously for several months on a laboratory scale with minimal maintenance requirements. The insect cells grow to high density and express concentrated functional recombinant protein in a small volume, simplifying and economizing downstream purification.