Premium
Micropatterned avidin arrays on silicon substrates via photolithography, self‐assembly and bioconjugation
Author(s) -
Vail Timothy L.,
Cushing Kevin W.,
Ingram Jani C.,
Omer Ingrid St.
Publication year - 2006
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20050085
Subject(s) - ethylene glycol , contact angle , micropatterning , bioconjugation , silane , microcontact printing , nanotechnology , biosensor , self assembled monolayer , biotinylation , chemistry , materials science , octadecyltrichlorosilane , self assembly , fourier transform infrared spectroscopy , monolayer , chemical engineering , organic chemistry , biochemistry , composite material , engineering
Processes for micropatterning protein arrays on inorganic substrates have gained attention in the development of biosensors and clinical diagnostics. This study demonstrates a chemically selective method based on photolithographic deposition of gold patterns with the subsequent attachment of functionalized alkanethiols via molecular self‐assembly. Selective capping of carboxy groups on alkanethiols by N ‐hydroxysuccinimide esters allowed the deposition of methoxypoly(ethylene glycol) silane as a blocking agent on unpatterned regions. Carboxylates were used to couple a form of avidin to create a microarray of protein. This microarray was successfully probed with biotinylated quantum dots. In‐process characterization methods included grazing‐angle Fourier‐transform IR spectroscopy, ellipsometry, contact‐angle goniometry, atomic‐force microscopy and fluorescence microscopy.