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Electrochemical studies on polysorbate‐20 (Tween 20)‐entrapped haemoglobin and its application in a hydrogen peroxide biosensor
Author(s) -
Ma Xiang,
Chen Ting,
Liu Lifang,
Li Genxi
Publication year - 2005
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20040189
Subject(s) - hydrogen peroxide , electrochemistry , biosensor , chemistry , polysorbate , chromatography , polyvinyl alcohol , combinatorial chemistry , organic chemistry , biochemistry , electrode , pulmonary surfactant
Haemoglobin (Hb) was entrapped in polysorbate 20 and then modified on a pyrolytic graphite electrode. Electrochemical studies revealed that a pair of stable and well‐defined redox peaks attributed to the direct redox reaction of Hb could be observed in a phosphate buffer solution (pH 6.0). The anodic and cathodic peaks were located at −236 and −316 mV (versus a saturated calomel reference electrode) separately. The formal potential, E 0 ′, was linearly varied with pH in the range from 3.0 to 10.0 with a slope of −48.0 mV·pH −1 . Moreover, the protein was capable of catalysing the reduction of H 2 O 2 . Accordingly, an unmediated biosensor for H 2 O 2 was prepared with a linear range from 8.0×10 −7 to 1.0×10 −3 M. This biosensor exhibited good stability, sensitivity and reproducibility.