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Fed‐batch pediocin production by Pediococcus acidilactici NRRL B‐5627 on whey
Author(s) -
Pérez Guerra Nelson,
Bernárdez Paula Fajardo,
Agrasar Ana Torrado,
López Macías Cristina,
Castro Lorenzo Pastrana
Publication year - 2005
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20040146
Subject(s) - pediococcus acidilactici , fermentation , food science , yeast extract , chemistry , bacteriocin , pediococcus , lactic acid , yeast , acetic acid , lactobacillus , biochemistry , bacteria , biology , antimicrobial , lactobacillus plantarum , organic chemistry , genetics
Cell growth and pediocin production by Pediococcus acidilactici NRRL B‐5627 on whey were compared by using batch fermentation and re‐alkalized fed‐batch fermentation. The batch fermentations were performed on DWG [DW (diluted whey) supplemented with 1% (w/v) glucose], DWYE [DW supplemented with 2% (w/v) yeast extract] and DWGYE (DW supplemented with 1% glucose plus 2% yeast extract) media. The fed‐batch culture on DWYE medium was fed with a mixture of concentrated whey (48 g of total sugars/l) supplemented with 2% yeast extract and 400 g/l concentrated glucose. The re‐alkalized fed‐batch culture was characterized by higher biomass (6.57 g/l) and pediocin [517.6 BU (bacteriocin activity units)/ml] concentrations compared with the batch processes on MRS (de Man, Rogosa and Sharpe) broth (1.76 g/l and 493.2 BU/ml), DW (0.17 g/l and 57.7 BU/ml), DWG (0.14 g/l and 53.6 BU/ml), DWYE (1.43 g/l and 187.6 BU/ml) and DWGYE (1.28 g/l and 167.3 BU/ml) media. A mixed acid fermentation was observed during the growth of P. acidilactici NRRL B‐5627 in the fed‐batch culture on DWYE medium, and other products (acetic acid and ethanol) in addition to lactic acid accumulated in the medium. Mathematical models were set up to describe fed‐batch production of biomass and pediocin by P. acidilactici . The models developed offer a better fit and a more realistic description of the experimental biomass and pediocin production data when compared with the logistic and Luedeking and Piret [(1959) J. Biochem. Microbiol. Technol. Eng. 1, 431–459] model.