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Two mathematical models for the correction of carbohydrate and protein interference in the determination of uronic acids by the m ‐hydroxydiphenyl method
Author(s) -
Murado Miguel Anxo,
Vázquez José Antonio,
Montemayor María Ignacia,
Cabo Marta López,
González María del Pilar
Publication year - 2005
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20040127
Subject(s) - uronic acid , chemistry , interference (communication) , biological system , carbohydrate , chromatography , biochemistry , computer science , polysaccharide , biology , computer network , channel (broadcasting)
The most common method in the routine determination of uronic acids, the m ‐hydroxydiphenyl reaction, recently adapted to rapid microplate analysis, has as a main inconvenience, in any one of their modalities, interferences due to the frequent presence of proteins and neutral carbohydrates in the samples. Corresponding corrections in the literature are unsatisfactory when applied to complex matrices, and further adaptation to the microplate analysis is not free from additional problems. With particular reference to hyaluronic acid, the interactions between the principal reactants and the interfering materials are studied kinetically under realistic conditions, and simple mathematical models are proposed which satisfactorily describe the experimental results and allow adequate corrections to be made.