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The combined effects of two anti‐aggregatory agents, α‐cyclodextrin and Ca 2+ , on the refolding process of denatured α‐amylase
Author(s) -
Yazdanparast Razieh,
Khodarahmi Reza
Publication year - 2005
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20040070
Subject(s) - chemistry , cyclodextrin , amylase , guanidinium chloride , enzyme , folding (dsp implementation) , chromatography , biochemistry , electrical engineering , engineering
It is now a well‐known fact that α‐CD (α‐cyclodextrin) and Ca 2+ are two important folding agents in the re‐activation and refolding processes of many denatured enzyme solutions. Bacillus sp. α‐amylase was extensively denatured in a 6 M guanidinium chloride solution overnight. Under suitable renaturation conditions, 20–30% more activity was recovered in the presence of various concentrations of α‐CD (0–100 mM). Similarly, 25–30% more activity was recovered under the influence of different concentrations of Ca 2+ (0–100 mM). Regardless of these results, both α‐CD and Ca 2+ at 100 mM concentrations were separately capable of preventing the extent of aggregate formation by 86 and 77% respectively. These observations clearly indicate that the effects of α‐CD and/or Ca 2+ on the process of aggregation and refolding (activity recovery) are not synchronized: both agents mostly act as anti‐aggregatory agents instead of acting as refolding agents.