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Optimization of penicillin G acylase multipoint immobilization on to glutaraldehyde–chitosan beads
Author(s) -
Adriano Wellington S.,
Filho Edilson H. C.,
Silva James A.,
Gonçalves Luciana R. B.
Publication year - 2005
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20040061
Subject(s) - glutaraldehyde , phenylacetic acid , chemistry , chitosan , penicillin amidase , sodium borohydride , chromatography , yield (engineering) , immobilized enzyme , nuclear chemistry , enzyme , biochemistry , materials science , catalysis , metallurgy
The objective of this work was to study the immobilization of penicillin G acylase from Escherichia coli on to chitosan–glutaraldehyde beads by multipoint covalent binding. This process was optimized using a 2 3 experimental design. The parameters selected for the present study were the concentrations of glutaraldehyde, phenylacetic acid and sodium borohydride. Three responses were chosen, namely immobilization yield and stabilization factors of enzyme derivatives at high temperature and at alkaline pH. All the runs at the maximum (+1) and minimum (−1) levels were performed at random. Three experiments were performed at the centre point, coded as zero, for experimental‐error estimation. With respect to immobilization yield, the main effectors were the concentrations of glutaraldehyde and phenylacetic acid. For stabilization factors at 50°C and at alkaline pH, the main effectors were the concentrations of glutaraldehyde and sodium borohydride and the interaction between them.