An efficient isocratic separation of hydroxycinnamates and their corresponding benzoates from microbial and plant sources by HPLC | Zendy

Sachan Ashish | Zendy; Ghosh Shashwati | Zendy; Mitra Adinpunya | Zendy

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An efficient isocratic separation of hydroxycinnamates and their corresponding benzoates from microbial and plant sources by HPLC

Author(s) -

Sachan Ashish,

Ghosh Shashwati,

Mitra Adinpunya

Publication year - 2004

Publication title -

biotechnology and applied biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.468

H-Index - 70

eISSN - 1470-8744

pISSN - 0885-4513

DOI - 10.1042/ba20030224

Subject(s) - vanillic acid , chemistry , chromatography , vanillin , trifluoroacetic acid , high performance liquid chromatography , ferulic acid , elution , benzoates , resolution (logic) , methanol , organic chemistry , artificial intelligence , computer science

A rapid HPLC‐based separation method was developed to analyse phenolic flavour components. In the present study, ferulic acid, 4‐coumaric acid, 4‐hydroxybenzoic acid, 4‐hydroxybenzaldehyde, vanillic acid and vanillin were chromatographed on various C 18 columns (Prodigy™ ODS2, Synergi™ Hydro‐RP, Lichrosorb® and Columbus™). A dual‐wavelength UV detector was used for the precise identification of the eluted components. An isocratic elution with aqueous trifluoroacetic acid (1 mM)/methanol (17:8) at a flow rate of 1.0 ml/min separated all the above six phenolic compounds within 21 min on a C 18 reverse‐phase column (Synergi™ Hydro‐RP) with stable baseline resolution.

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