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Development of an adsorption procedure for the direct separation and purification of prodigiosin from culture broth
Author(s) -
Wang Xuedong,
Tao Jinli,
Wei Dongzhi,
Shen Yaling,
Tong Wangyu
Publication year - 2004
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20030210
Subject(s) - prodigiosin , adsorption , chromatography , chemistry , expanded bed adsorption , pulmonary surfactant , yield (engineering) , serratia marcescens , materials science , organic chemistry , biochemistry , escherichia coli , metallurgy , gene
A novel procedure for the direct separation and purification of prodigiosin based on adsorption chromatography is provided. A surfactant (Tween 80; 0.1%, w/v) was used to improve prodigiosin release from the cell envelope. An adsorbent (X‐5 resin) with high loading capacity (7.3 mg/g) was selected for the direct recovery of prodigiosin from culture broth by static adsorption or in situ separation. This technology not only simplified the separation process, but also made the recovery yield (83%) much higher when compared with the conventional method (50%). As a result, it will be particularly suited for large‐scale separation and purification of prodigiosin.