Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research | Zendy

Jacquot JeanPierre | Zendy; Rouhier Nicolas | Zendy; Navrot Nicolas | Zendy; Decottignies JeanPierre | Zendy

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Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research

Author(s) -

Jacquot JeanPierre,

Rouhier Nicolas,

Navrot Nicolas,

Decottignies JeanPierre

Publication year - 2004

Publication title -

biotechnology and applied biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.468

H-Index - 70

eISSN - 1470-8744

pISSN - 0885-4513

DOI - 10.1042/ba20030196

Subject(s) - polyclonal antibodies , thioredoxin , antibody , western blot , microbiology and biotechnology , biochemistry , antigen , chemistry , sepharose , biology , enzyme , immunology , gene

A single rabbit has been immunized with a mixture of six different proteins: green fluorescent protein, ferredoxin:NADP oxidoreductase, NADPH:thioredoxin reductase, thioredoxin f , fructose bisphosphatase and cyclophilin. Individual IgGs were re‐purified from the serum through specific interaction on small columns generated by grafting the antigen on a CNBr‐Sepharose matrix. The specificity of the isolated IgG was checked by Western‐blot analysis. Purified and specific IgG fractions were obtained with all antigens except thioredoxin f .

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