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Evaluation of selected control strategies for fed‐batch cultures of a hybridoma cell line
Author(s) -
Pörtner Ralf,
Schwabe JanOliver,
Frahm Björn
Publication year - 2004
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20030168
Subject(s) - computer science , process (computing) , a priori and a posteriori , biochemical engineering , production (economics) , adaptive control , process engineering , control (management) , production line , fed batch culture , process control , control theory (sociology) , biology , fermentation , artificial intelligence , biochemistry , engineering , mechanical engineering , philosophy , epistemology , economics , macroeconomics , operating system
While fed‐batch suspension culture of animal cells continues to be of industrial importance for the large‐scale production of pharmaceutical products, existing control concepts are still insufficient. The present paper illustrates the advantages and disadvantages of different fed‐batch strategies, including fixed‐feed trajectories, control via OUR (oxygen uptake rate) (stoichiometric feeding), a priori determination of feed trajectories based on a kinetic model and the model‐based adaptive OLFO (open‐loop‐feedback‐optimal) control strategy. A recommendation as to which control strategy should be used for a specific process has to consider the respective process. For an established process with a well characterized and stable production cell line, probably the application of a fixed feed trajectory should be recommended. An adaptive, model‐based control strategy could be the method of choice during cell‐line development or for rapid production of small amounts of product for clinical trials, owing to its universal character and because it does not require intensive process development.