Premium
Purification of goat immunoglobulin G by immobilized metal‐ion affinity using cross‐linked alginate beads
Author(s) -
Jain Sulakshana,
Gupta Munishwar N.
Publication year - 2004
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20030139
Subject(s) - epichlorohydrin , chemistry , chromatography , metal , metal ions in aqueous solution , polymer , polymer chemistry , organic chemistry
The alginate beads obtained by cross‐linking of the polymer by epichlorohydrin were charged with Cu(II). The copper‐charged beads could be directly used as a immobilized‐metal‐affinity‐chromatographic medium for purification of goat IgG. The best results in the packed‐bed mode were obtained by using beads charged with 95.4 μmol/ml Cu(II). We found that we could recover 97.4% IgG with an 8‐fold purification.