Purification of goat immunoglobulin G by immobilized metal‐ion affinity using cross‐linked alginate beads | Zendy

Jain Sulakshana | Zendy; Gupta Munishwar N. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Purification of goat immunoglobulin G by immobilized metal‐ion affinity using cross‐linked alginate beads

Author(s) -

Jain Sulakshana,

Gupta Munishwar N.

Publication year - 2004

Publication title -

biotechnology and applied biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.468

H-Index - 70

eISSN - 1470-8744

pISSN - 0885-4513

DOI - 10.1042/ba20030139

Subject(s) - epichlorohydrin , chemistry , chromatography , metal , metal ions in aqueous solution , polymer , polymer chemistry , organic chemistry

The alginate beads obtained by cross‐linking of the polymer by epichlorohydrin were charged with Cu(II). The copper‐charged beads could be directly used as a immobilized‐metal‐affinity‐chromatographic medium for purification of goat IgG. The best results in the packed‐bed mode were obtained by using beads charged with 95.4 μmol/ml Cu(II). We found that we could recover 97.4% IgG with an 8‐fold purification.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research