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Biological effect of radiation‐degraded alginate on flower plants in tissue culture
Author(s) -
Luan Le Q.,
Hien Nguyen Q.,
Nagasawa Naotsugu,
Kume Tamikazu,
Yoshii Fumio,
Nakanishi Tomoko M.
Publication year - 2003
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20030058
Subject(s) - plantlet , shoot , horticulture , irradiation , botany , tissue culture , biology , greenhouse , chemistry , in vitro , biochemistry , physics , nuclear physics
Alginate with a weight‐average molecular mass ( M w ) of approx. 9.04×10 5 Da was irradiated at 10–200 kGy in 4% (w/v) aqueous solution. The degraded alginate product was used to study its effectiveness as a growth promoter for plants in tissue culture. Alginate irradiated at 75 kGy with an M w of approx. 1.43×10 4 Da had the highest positive effect in the growth of flower plants, namely limonium, lisianthus and chrysanthemum. Treatment of plants with irradiated alginate at concentrations of 30–200 mg/l increased the shoot multiplication rate from 17.5 to 40.5% compared with control. In plantlet culture, 100 mg/l irradiated alginate supplementation enhanced shoot height (9.7–23.2%), root length (9.7–39.4%) and fresh biomass (8.1–19.4%) of chrysanthemum, lisianthus and limonium compared with that of the untreated control. The survival ratios of the transferred flower plantlets treated with irradiated alginate were almost the same as the control value under greenhouse conditions. However, better growth was attained for the treated plantlets.