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Modified alginate matrices for the immobilization of bioactive agents
Author(s) -
LeTien Canh,
Millette Mathieu,
Lacroix Monique,
Mateescu MirceaAlexandru
Publication year - 2004
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20030054
Subject(s) - chemistry , nisin , immobilized enzyme , substrate (aquarium) , enzyme assay , enzyme , bacteriocin , chromatography , yield (engineering) , biochemistry , organic chemistry , biology , materials science , ecology , metallurgy , antimicrobial
Bioactive agents (catalase – an enzyme, and nisin – a bacteriocin) were covalently immobilized on alginate activated with sodium periodate (oxidatively converting 2,3‐dihydroxy groups into dialdehyde residues), followed or preceded by ionotropic gelation. For the same protein coupling yield, the retained enzyme activity of the immobilized enzyme (ImE) can be markedly increased by diminishing the bead diameter, a phenomenon that illustrates the role of substrate/product diffusion through the bead gel layer. When the amount of enzyme introduced for coupling was about 15 mg/100 mg of support and the bead diameter was about 100 μm, a high retained specific activity (95–98%) was obtained. Diffusion phenomena can be markedly decreased by enzyme immobilization on the surface of microbeads (obtained by gelation of activated alginate prior to immobilization). In this case, the retained activity was approx. 75% of that of the free enzyme. A slightly higher K m value of ImE suggested that the enzyme–substrate affinity was almost maintained. The profiles of ImE activities at various pH values, at various temperatures and when undergoing proteolysis showed a overall higher stability for the immbolized than that for the free enzyme. Nisin immobilized on the microbead surface, when submitted to proteolysis, conserved its bacteriocin activity, strongly inhibiting the growth of Lactobacillus sake when subjected to an agar spot test, whereas free nisin totally lost its activity.