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Lipase‐catalysed transesterification of soya bean oil for biodiesel production during continuous batch operation
Author(s) -
Du Wei,
Xu Yuanyuan,
Liu Dehua
Publication year - 2003
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20030032
Subject(s) - transesterification , lipase , glycerol , chemistry , alcohol , biodiesel , triacylglycerol lipase , biodiesel production , interesterified fat , molar ratio , chromatography , organic chemistry , catalysis , enzyme
The effects of temperature, oil/alcohol molar ratio and by‐product glycerol were studied during Lipozyme TL IM‐catalysed continuous batch operation when short‐chain alcohols were used as the acyl acceptor. In non‐continuous batch operation, the optimal oil/alcohol ratio and temperature were 1:4 and 40–50 °C; however, during the continuous batch operation, the optimal oil/alcohol ratio and temperature were 1:1 and 30 °C; 95% of enzymic activity remained after 10 batches when isopropanol was adopted to remove by‐product glycerol during repeated use of the lipase.