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A strategic study using mutant‐strain entrapment in calcium alginate for the production of Saccharomyces cerevisiae cells with high invertase activity
Author(s) -
RossiAlva Juan Carlos,
RochaLeão Maria Helena Miguez
Publication year - 2003
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20020101
Subject(s) - invertase , sucrose , saccharomyces cerevisiae , calcium alginate , yeast , chemistry , biochemistry , calcium , sugar , bioconversion , fermentation , organic chemistry
Entrapped cells and entrapped cells grown inside of a calcium alginate matrix as well as free cells have been investigated using Saccharomyces cerevisiae mutant strains with regard to their pattern of growth and invertase activity. The repression of invertase by glucose and glucose‐consumption ability were considered in the selection process of the mutants. Efficient sucrose bioconversion due to high invertase activity was obtained when entrapped mutant strain Q6R2 cells were grown within calcium alginate gel beads using sucrose plus glucose as the carbon source. Under these conditions, 1 mg (dry weight) of entrapped cells is able to produce 20 μmol of inverted sugar in 3 min (the maximum activity obtained was 20 units·mg −1 ). The experiments were carried out for 6 months without appreciable loss of either bead integrity or invertase activity. The biocatalyst was also stored at 4 °C for 6 months without appreciable loss of the invertase activity. This work shows that entrapped yeast cells with a weak ability to consume sugar may be used to produce inverted sugar.