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A simplified method for identification of human cardiac myosin heavy‐chain isoforms
Author(s) -
Piao Shengfu,
Yu Fushun,
Mihm Michael J.,
Reiser Peter J.,
McCarthy Patrick M.,
Van Wagoner David R.,
Bauer John Anthony
Publication year - 2003
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20020076
Subject(s) - myosin , gene isoform , myh7 , biology , myocyte , cardiac myocyte , atp hydrolysis , major histocompatibility complex , muscle contraction , microbiology and biotechnology , atpase , cardiac muscle , myosin light chain kinase , biochemistry , computational biology , biophysics , enzyme , anatomy , gene
Cardiac myosin is a central participant in the cross‐bridge cycling that mediates myocyte contraction and consists of multiple subunits that mediate both hydrolysis of ATP and mechanical production of contractile force Two isoforms of myosin heavy chain (MHC‐ α and MHC‐ β ) are known to exist in mammalian cardiac tissue, and it is within this myosin subunit that ATPase activity resides. These isoforms differ by less than 0.2% in total molecular mass and amino acid sequence, but, strikingly, influence the rate and efficiency of energy utilization for generation of contractile force. Changes in the MHC‐ α /MHC‐ β ratio has been classically viewed as an adaptation of a failing myocyte in both animal models and humans; however, their measurement has traditionally required specialized preparations and materials for sufficient resolution. Here we describe a greatly simplified method for routine assessments of myosin isoform composition in human cardiac tissues. The primary advantages of our approach include higher throughput and reduced supply costs with no apparent loss of statistical power, reproducibility or achieved results. Use of this more convenient method may provide enhanced access to an otherwise specialized technique and could provide additional opportunity for investigation of cardiac myocyte adaptive changes.