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Production of bacteriocins from Lactococcus lactis subsp. lactis CECT 539 and Pediococcus acidilactici NRRL B‐5627 using mussel‐processing wastes
Author(s) -
Guerra Nelson Pérez,
Castro Lorenzo Pastrana
Publication year - 2002
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20020031
Subject(s) - pediococcus acidilactici , lactococcus lactis , bacteriocin , nisin , pediococcus , food science , chemistry , bacteria , mussel , lactic acid , microbiology and biotechnology , biology , lactobacillus , fermentation , fishery , organic chemistry , genetics , antimicrobial , lactobacillus plantarum
The growth and bacteriocin production by Lactococcus lactis subsp. lactis CECT 539 and Pediococcus acidilactici NRRL B‐5627 were investigated on mussel‐processing wastes. Both bacteriocin productions were satisfactorily modelled using a modified form of the Luedeking and Piret expression, which includes a term for the influence of the pH reduction rate. Experimental data from cultures buffered at different initial concentrations (0, 0.03, 0.10 and 0.25 M) of both bacteria were used to fit and verify the model. The influence of total sugars, nitrogen, phosphorus and buffer concentration on nisin and pediocin production was also studied using response‐surface methodology and empirical modelling. Enhanced nisin production (33 BU/ml) was achieved in media buffered with 0.10 M potassium hydrogen phthalate/NaOH. However, the highest levels of pediocin (368 BU/ml) were obtained in the non‐buffered media.