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Development of an optimized, simple chemically defined medium for bacterial cellulose production by Acetobacter sp. A9 in shaking cultures
Author(s) -
Heo MoonSoo,
Son HongJoo
Publication year - 2002
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20020018
Subject(s) - acetobacter , cellulose , bacterial cellulose , nuclear chemistry , chemistry , bacteria , ethanol , biochemistry , fermentation , biology , genetics
The genus Acetobacter can synthesize cellulose when grown in an undefined medium containing glucose. By using the technique of the omission of a single medium component, an optimized and simple chemically defined medium was developed to support cellulose production by Acetobacter sp. A9 in shaking culture. It contained 4.0% (w/v) glucose, 0.2% (w/v) (NH 4 ) 2 SO 4 , 0.25% (w/v) KH 2 PO 4 , 0.3% (w/v) Na 2 HPO 4 ·12H 2 O, 0.05% (w/v) MgSO 4 ·7H 2 O, 0.0002% (w/v) FeSO 4 ·7H 2 O, 0.00025% (w/v) H 3 BO 3 , 0.00006% (w/v) nicotinamide, 0.00025% (w/v) inositol and 1.4% (v/v) ethanol. A maximum cellulose concentration of around 8 g/l was achieved after 9 days of cultivation at 200 rev./min. The production of cellulose by Acetobacter sp. A9 was greater in simplified synthetic medium than in complex medium (Hestrin and Schramm medium) conventionally used for Acetobacter strains.