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Impaired l ‐arginine uptake in platelets from type‐2 diabetic patients
Author(s) -
Signorello Maria Grazia,
Giovine Marco,
Pascale Raffaele,
Cataldi Luigi,
Bordone Caterina,
Benatti Umberto,
Leoncini Giuliana
Publication year - 2001
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20010022
Subject(s) - arginine , platelet , intracellular , stimulation , endocrinology , medicine , chemistry , endogeny , biochemistry , amino acid
The l ‐arginine uptake of resting platelets from type‐2 diabetic patients and control subjects was measured and the kinetic parameters defined. The effect of platelet stimulation with agonists was also investigated. Kinetic studies showed that the K m value for l ‐arginine transport was not different in patients compared with control subjects, while V max was significantly decreased in patients compared with controls. Moreover, agonists able to mobilize Ca 2+ produced a further decrease in the l ‐arginine uptake in controls and in patients, suggesting that Ca 2+ intracellular levels down‐regulate l ‐arginine transport in platelets from both control subjects and patients. Data suggest that drugs designed to control intracellular Ca 2+ might restore platelet function in diabetic patients.