Premium
Two telomerase reverse transcriptases (TERTs) expressed in Candida albicans
Author(s) -
Metz Anneke M.,
Love Ruschelle A.,
Strobel Gary A.,
Long David M.
Publication year - 2001
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20010021
Subject(s) - biology , gene , candida albicans , reverse transcriptase , telomerase , genetics , saccharomyces cerevisiae , telomerase reverse transcriptase , yeast , telomere , microbiology and biotechnology , polymerase chain reaction
The human pathogenic yeast Candida albicans contains two telomerase reverse transcriptase ( TERT ) genes. CaTERT1 and CaTERT2 appear either to be two alleles of the same gene or two entirely different genes that encode 867‐residue proteins that differ by five amino acids. Both TERTs have a calculated pI of 9·5 and a M r of 100·9 kDa and are the smallest TERTs identified to date. Both genes appear to be expressed. Based on sequence similarity between CaTERT1 and the Saccharomyces cerevisiae orthologue Est2p, we suggest a revised alignment for motif E of Est2p. The identification of these TERT genes provides the first opportunity to study telomerase in an important human pathogen.