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Purification of alkaline phosphatase from chicken intestine by expanded‐bed affinity chromatography on dye‐linked cellulose
Author(s) -
Roy Ipsita,
Gupta Munishwar N.
Publication year - 2000
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20000021
Subject(s) - cellulose , chromatography , alkaline phosphatase , chemistry , affinity chromatography , enzyme , biochemistry
Alkaline phosphatase from chicken intestine was purified on dye‐linked cellulose beads. The various dye‐linked cellulose beads were screened in the batch mode for purification of the enzyme and the promising dyes were tried in the expanded‐bed mode. The one‐step expanded‐bed affinity chromatography on Cibacron Blue‐linked cellulose beads yielded 48‐fold purification with an activity recovery of 70%.