Premium
Purification and characterization of biosurfactants from Nocardia sp. L‐417
Author(s) -
Kim Soon Han,
Lim Ee Jong,
Lee Sang Ok,
Lee Jae Dong,
Lee Tae Ho
Publication year - 2000
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba19990111
Subject(s) - chromatography , sephadex , size exclusion chromatography , fractionation , acetone , chemistry , silica gel , emulsion , pulmonary surfactant , ammonium , gel permeation chromatography , hexane , hexadecane , organic chemistry , enzyme , biochemistry , polymer
The Nocardia sp. L‐417 strain grown with n ‐hexadecane as a carbon source produced two types of biosurfactant that have different characteristics. These biosurfactants were purified by procedures that included ammonium sulphate fractionation, chilled acetone and hexane treatments, silica‐gel column chromatography and Sephadex LH‐20 gel filtration. The purified biosurfactants were very stable over a broad range of pHs (2–12) and temperatures (100 °C, 3 h). The biosurfactant type I had strong properties as an emulsifying agent and as an emulsion‐stabilizing agent, whereas type II had a strong ability to reduce surface tension.