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An evaluation of hybridization kinetics in biosensors using a single‐fractal analysis
Author(s) -
Vontel Sunita,
Ramakrishnan Anand,
Sadana Ajit
Publication year - 2000
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba19990092
Subject(s) - analyte , biosensor , fractal dimension , fractal analysis , fractal , diffusion , kinetics , biological system , receptor–ligand kinetics , chemistry , analytical chemistry (journal) , materials science , nanotechnology , chromatography , mathematics , physics , thermodynamics , biology , mathematical analysis , quantum mechanics
The diffusion‐limited hybridization kinetics of analyte in solution to receptor immobilized on a biosensor or immunosensor surface is analysed within a fractal framework. The data may be analysed by a single‐fractal analysis. This was indicated by the regression analysis provided by Sigmaplot [Sigmaplot Users Manual (1993) Jandel Scientific, San Rafael, CA]. It is of interest to note that the binding‐rate coefficient and the fractal dimension both exhibit changes in the same and in the opposite directions for the single example presented in each case. The binding‐rate coefficient(s) expressions developed as a function of the analyte (DNA) concentration in solution and the fractal dimension are of particular value, since they provide a means to better control biosensor or immunosensor performance and provide physical insights into the hybridization process.