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Polyclonal‐antibody‐mediated insolubilization and stabilization of papain
Author(s) -
Khan Shakil A.,
Iqbal Jawaid
Publication year - 2000
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba19990091
Subject(s) - papain , antiserum , polyclonal antibodies , iodoacetamide , chemistry , iodoacetic acid , antibody , enzyme , biochemistry , chromatography , trypsin , microbiology and biotechnology , cysteine , biology , immunology
Antisera raised in rabbits against native iodoacetamide‐ and iodoacetic acid‐modified papain contained precipitating and non‐inhibitory antibodies. Papain could be insolubilized as enzyme–antibody adducts with the γ‐globulin fraction derived from the antiserum. Two insolubilized papain preparations, designated A and B, with low and high antibody/enzyme ratios, respectively, exhibited high enzyme activity and markedly enhanced thermal and denaturant stabilities. However, papain antibody adduct B was superior in activity and stability over preparation A. The usefulness of immobilized papain in biopharmaceutical and other industries is also discussed.