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Affinity adsorbents for the vancomycin group of antibiotics
Author(s) -
Yan Husheng,
Zhao Qingxiang,
Yuan Jing,
Cheng Xiaohui,
He Binglin
Publication year - 2000
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba19990039
Subject(s) - adsorption , peptidoglycan , vancomycin , chemistry , aqueous solution , antibiotics , glycopeptide , nuclear chemistry , acetonitrile , bacteria , cell wall , chromatography , organic chemistry , biochemistry , staphylococcus aureus , biology , genetics
The vancomycin group of antibiotics kill Gram‐positive bacteria by binding to nascent bacterial cell‐wall peptidoglycan bearing the C‐terminal sequence‐D‐Ala‐D‐Ala. In this paper, affinity adsorbents for the vancomycin group of antibiotics were prepared by immobilizing the peptidoglycan analogues ‐D‐Ala‐D‐Ala, ‐succinyl‐D‐Ala and ‐succinyl‐Gly on to crosslinked poly( N,N ‐dimethylacrylamide). The adsorption capacities of the three adsorbents for demethylvancomycin were 0.59, 0.35 and 0.29 mmol/g, respectively. The adsorption capacity of the adsorbent with‐D‐Ala‐D‐Ala for vancomycin was 0.53 mmol/g. In contrast, the adsorbent bearing ‐succinyl‐L‐Ala hardly adsorbed demethylvancomycin. Aqueous sodium carbonate (0.4 M)/acetonitrile (7/3, v/v) completely desorbed demethylvancomycin adsorbed on the adsorbents.