Open AccessCellular point-of-care diagnostics using an inexpensive layer-stack microfluidic device
Author(s) -
Kilean Lucas,
Juhyun Oh,
Jan Hoelzl,
Ralph Weissleder
Publication year - 2022
Publication title -
lab on a chip
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.064
H-Index - 210
eISSN - 1473-0197
pISSN - 1473-0189
DOI - 10.1039/d2lc00162d
Subject(s) - bottleneck , stack (abstract data type) , microfluidics , point of care , multiplexing , computer science , layer (electronics) , nanotechnology , computer hardware , materials science , embedded system , medicine , telecommunications , pathology , programming language
Cellular analyses are increasingly used to diagnose diseases at point-of-care and global healthcare settings. Some analyses are simple as they rely on chromogenic stains (blood counts, malaria) but others often require higher multiplexing to define and quantitate cell populations (cancer diagnosis, immunoprofiling). Simplifying the latter with inexpensive solutions represents a current bottleneck in designing start-end pipelines. Based on the hypothesis that novel film adhesives could be used to create inexpensive disposable devices, we tested a number of different designs and materials, to rapidly perform 12-15 channel single-cell imaging. Using an optimized passive pumping layer-stack microfluidic (PLASMIC) device (<1 $ in supplies) we show that rapid, inexpensive cellular analysis is feasible.