Open AccessExtensive heterogeneity of glycopeptides in plasma revealed by deep glycoproteomic analysis using size-exclusion chromatography
Author(s) -
Mayank Saraswat,
Kishore Garapati,
Dong-Gi Mun,
Akhilesh Pandey
Publication year - 2021
Publication title -
molecular omics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 10
ISSN - 2515-4184
DOI - 10.1039/d1mo00132a
Subject(s) - glycoproteomics , glycopeptide , glycoprotein , glycan , glycosylation , proteome , chemistry , sialic acid , biochemistry , proteomics , epitope , computational biology , biology , antigen , gene , immunology , antibiotics
Size-exclusion chromatography facilitates simultaneous enrichment and fractionation of N -glycopeptides. Combining it with improved LC-MS/MS analysis achieves deep coverage of plasma N -glycoproteome.