Open AccessProximity-enhanced synthesis of DNA–peptide–DNA triblock molecules
Author(s) -
T. MacCulloch,
Alexandra Novacek,
Nicholas Stephanopoulos
Publication year - 2022
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/d1cc04970d
Subject(s) - oligonucleotide , dna , bioorthogonal chemistry , peptide , combinatorial chemistry , chemistry , computational biology , biochemistry , biology , click chemistry
We report a proximity-enhanced method to synthesize a peptide flanked by two different oligonucleotide handles. Our method relies on sequential bioorthogonal reactions, and partial hybridization of the second handle to the first. We demonstrate the synthesis of a protease-responsive DNA "latch" and a cyclic bioactive peptide using this method.