Open AccessExcimer–monomer fluorescence changes by supramolecular disassembly for protein sensing and quantification
Author(s) -
Hongxu Liu,
Jenna Westley,
S. Thayumanavan
Publication year - 2021
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/d1cc03944j
Subject(s) - excimer , monomer , supramolecular chemistry , fluorescence , dissociation (chemistry) , selectivity , chemistry , nanotechnology , materials science , molecule , polymer , organic chemistry , physics , quantum mechanics , catalysis
A protein binding-induced supramolecular dissociation strategy is developed with the ratio of monomer and excimer fluorescence as the tool for protein sensing and quantification. Due to the "lock-and-key" strategy based on specific ligand-protein binding, the probe exhibits excellent selectivity and quantification accuracy to the protein of interest. The ratiometric approach is immune to interference from extrinsic quenchers, while preserving the opportunity to be protein specific.