Open AccessNanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing
Author(s) -
Bankala Krishnarjuna,
Toshio Yamazaki,
G.M. Anantharamaiah,
Ayyalusamy Ramamoorthy
Publication year - 2021
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/d1cc01018b
Subject(s) - flavin mononucleotide , flavin group , chemistry , reductase , flavoprotein , stereochemistry , biochemistry , enzyme
Cytochrome-P450-reductase transfers electrons to cytochrome-P450 through its flavin mononucleotide binding domain (FBD). Despite the importance of membrane-anchoring for FBD function, studies have focused on its soluble domain lacking the transmembrane-domain. Here we demonstrate that the reconstitution of FBD in nanodiscs enables high-resolution NMR measurements and renders a stable conformation.