Open AccessDevelopment of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid
Author(s) -
Can Zhang,
Xiaoxiao Wu,
Dongyang Li,
Jinnuo Hu,
Debin Wan,
Zhen Zhang,
Bruce D. Hammock
Publication year - 2021
Publication title -
analytical methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.615
H-Index - 67
eISSN - 1759-9679
pISSN - 1759-9660
DOI - 10.1039/d1ay00129a
Subject(s) - immunoassay , detection limit , chromatography , metabolite , chemistry , urine , mass spectrometry , antibody , biochemistry , biology , immunology
As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow immunoassay for detecting 3-PBA. The limit of detection (LOD) values for detecting 3-PBA by flow-through dot ELISA and GNP lateral-flow immunoassay were 0.01 ng mL -1 and 0.1 ng mL -1 , respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA.