Open AccessA near-infrared fluorescent probe based on a hemicyanine dye with an oxazolidine switch for mitochondrial pH detection
Author(s) -
Wafa Mazi,
Yunnan Yan,
Yibin Zhang,
Shuai Xia,
Shulin Wan,
Momoko Tajiri,
Rudy L. Luck,
Haiying Liu
Publication year - 2021
Publication title -
journal of materials chemistry. b
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 101
eISSN - 2050-7518
pISSN - 2050-750X
DOI - 10.1039/d0tb02181d
Subject(s) - fluorescence , oxazolidine , infrared , photochemistry , near infrared spectroscopy , materials science , chemistry , biophysics , organic chemistry , optics , biology , physics
A near-infrared fluorescent probe (AH+) has been prepared by incorporating an oxazolidine switch into a near-infrared hemicyanine dye. The probe shows fast and sensitive responses to pH from an oxazolidine switch to the hemicyanine dye upon pH decreases from 10.0 to 5.0. The probe shows good photostability, low cytotoxicity, and reversible fluorescence responses to pH changes with a pKa value of 7.6. It has been successfully used to determine pH changes in mitochondria.