Open AccessSimulation-directed amplifiable nanoparticle enhanced quantitative scattering assay under low magnification dark field microscopy
Author(s) -
Dali Sun,
Li Yang,
Christopher J. Lyon,
Tony Hu
Publication year - 2020
Publication title -
journal of materials chemistry. b
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 101
eISSN - 2050-7518
pISSN - 2050-750X
DOI - 10.1039/d0tb00350f
Subject(s) - magnification , materials science , dark field microscopy , scattering , linker , microscopy , field (mathematics) , nanoparticle , sensitivity (control systems) , signal (programming language) , analytical chemistry (journal) , nanotechnology , optics , chromatography , chemistry , physics , computer science , electronic engineering , mathematics , pure mathematics , engineering , programming language , operating system
Nanoparticle-enhanced assays read by high-magnification dark-field microscopy require time-intensive analysis methods subject to selection bias, which can be resolved by using low magnification dark-field assays (LMDFA), at the cost of reduced sensitivity. We have simulated and experimentally validated a tunable linker-based signal amplification strategy yielding 6-fold enhanced LMDFA sensitivity.