Open AccessModular control ofl -tryptophan isotopic substitutionviaan efficient biosynthetic cascade
Author(s) -
Clayton M. Thompson,
Allwin D. McDonald,
Hanming Yang,
Silvia Cavagnero,
Andrew R. Buller
Publication year - 2020
Publication title -
organic and biomolecular chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 146
eISSN - 1477-0539
pISSN - 1477-0520
DOI - 10.1039/d0ob00868k
Subject(s) - chemistry , cascade , substitution (logic) , modular design , tryptophan , combinatorial chemistry , biosynthesis , stereochemistry , biochemistry , enzyme , amino acid , chromatography , programming language , operating system , computer science
Isotopologs are powerful tools for investigating biological systems. We report a biosynthetic-cascade synthesis of Trp isotopologs starting from indole, glycine, and formaldehyde using the enzymes l-threonine aldolase and an engineered β-subunit of tryptophan synthase. This modular route to Trp isotopologs is simple and inexpensive, enabling facile access to these compounds.