Open AccessSecondary modification of oxidatively-modified proline N-termini for the construction of complex bioconjugates
Author(s) -
Johnathan C. Maza,
Alexandra V. Ramsey,
Meire Mehare,
Shane W. Krska,
Craig A. Parish,
Matthew B. Francis
Publication year - 2020
Publication title -
organic and biomolecular chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 146
eISSN - 1477-0539
pISSN - 1477-0520
DOI - 10.1039/d0ob00211a
Subject(s) - chemistry , proline , combinatorial chemistry , posttranslational modification , stereochemistry , biochemistry , amino acid , enzyme
A convenient two-step method is reported for the ligation of alkoxyamine- or hydrazine-bearing cargo to proline N-termini. Using this approach, bifunctional proline N-terminal bioconjugates are constructed and proline N-terminal proteins are immobilized.