Open AccessUnraveling dominant surface physicochemistry to build antimicrobial peptide coatings with supramolecular amphiphiles
Author(s) -
Zhou Ye,
Alexandra C. Kobe,
Ting Sang,
Conrado Aparicio
Publication year - 2020
Publication title -
nanoscale
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.038
H-Index - 224
eISSN - 2040-3372
pISSN - 2040-3364
DOI - 10.1039/d0nr04526h
Subject(s) - supramolecular chemistry , amphiphile , hydrogen bond , polarity (international relations) , antimicrobial , peptide , nanotechnology , materials science , supramolecular assembly , supramolecular chirality , chemistry , combinatorial chemistry , organic chemistry , molecule , copolymer , polymer , biochemistry , cell
With the increasing threat from antibiotic-resistant bacteria, surface modification with antimicrobial peptides (AMP) has been promisingly explored for preventing bacterial infections. Little is known about the critical factors that govern AMP-surface interactions to obtain stable and active coatings. Here, we systematically monitored the adsorption of a designer amphipathic AMP, GL13K, on model surfaces. Self-assembly of the GL13K peptides formed supramolecular amphiphiles that highly adsorbed on negatively charged, polar hydroxyapatite-coated sensors. We further tuned surface charge and/or surface polarity with self-assembled monolayers (SAMs) on Au sensors and studied their interactions with adsorbed GL13K. We determined that the surface polarity of the SAM-coated sensors instead of their surface charge was the dominant factor governing AMP/substrate interactions via hydrogen bonding. Our findings will instruct the universal design of efficient self-assembled AMP coatings on biomaterials, biomedical devices and/or natural tissues.